Highly productive alpha-amylases

ABSTRACT

The invention relates to mutant α-amylases that may be produced at high yield from recombinant microorganisms.

TECHNICAL FIELD

The present invention relates to mutant α-amylases having improved productivity.

BACKGROUND ART

α-Amylases [EC.3.2.1.1.] have been used in a wide range of industrial fields such as starch industry, brewing industry, fiber industry, pharmaceutical industry and food industry. Among them, those capable of degrading starches at high random are suited for detergents. Conventionally known as such are, as well as α-amylases derived from Bacillus licheniformis, liquefying alkaline α-amylases derived from the alkaliphilic strain Bacillus sp. KSM-AP1378 (FERM BP-3048) (WO94/26881) and improved enzymes having improved heat resistance and oxidant resistance (WO98/44126).

The present inventors have recently found liquefying alkaline α-amylases derived from the alkaliphilic strain Bacillus sp. KSM-K38 (FERM BP-6946) and having chelating-agent- and oxidation-resistance (Japanese Patent Application No. Hei 10-362487, Japanese Patent Application No. Hei 10-362488); and improved enzymes having improved heat resistance (Japanese Patent Application No. Hei 11-163569).

In addition to such properties, enzymes for detergents are required to have high productivity in consideration of their industrial production. Although various trials have been made to improve the heat resistance or oxidant resistance of α-amylases for detergent by using protein engineering technique, neither improvement of productivity has been considered sufficiently nor an attempt of production increase by mutation of a structural gene has been reported yet.

An object of the present invention is to provide mutant α-amylases having excellent productivity.

DISCLOSURE OF THE INVENTION

The present inventors introduced, in microorganisms, mutant α-amylase structural gene constructed by site-directed mutagenesis and evaluated productivity of α-amylases. As a result, it has been found that since an α-amylase gene has a site taking part in the improvement of productivity, introduction, into a microorganism, of a recombinant gene having this site mutated makes it possible to produce α-amylases having drastically improved productivity.

In one aspect of the present invention, there is thus provided a mutant α-amylase which is derived from an α-amylase having an amino acid sequence represented by SEQ ED No. 2 or showing at least 60% homology thereto by substitution or deletion of at least one amino acid residue corresponding to any one of Pro₁₈, Gln₈₆, Glu₁₃₀, Asn₁₅₄, Arg₁₇₁, Ala₁₈₆, Glu₂₁₂, Val₂₂₂, Tyr₂₄₃) Pro₂₆₀, Lys₂₆₉, Glu₂₇₆, Asn₂₇₇, Arg₃₁₀, Glu₃₆₀, Gln₃₉₁, Trp₄₃₉, Lys₄₄₄, Asn₄₇₁ and Gly₄₇₆ of the amino acid sequence.

In another aspect of the present invention, there is also provided a mutant α-amylase derived from an α-amylase having an amino acid sequence represented by SEQ ID No. 4 or showing at least 60% homology thereto by substitution or deletion of at least one amino acid residue corresponding to any one of Asp₁₂₈, Gly₁₄₀, Ser₁₄₄, Arg₁₆₈, Asn₁₈₁, Glu₂₀₇, Phe₂₇₂, Ser₃₇₅, Trp₄₃₄ and Glu₄₆₆ of the amino acid sequence.

In a further aspect of the present invention, there is also provided a gene encoding this mutant α-amylase, a vector containing the gene, a cell transformed with the vector and a production method of a mutant α-amylase which comprises cultivating the transformed cell.

In a still further aspect of the present invention, there is also provided a detergent composition containing this mutant α-amylase.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 illustrates a method of constructing a recombinant plasmid for production of an α-amylase derived from the strain KSM-1378 or KSM-K38.

FIG. 2 is a schematic view illustrating a method of introducing a mutation into an α-amylase gene derived from the strain KSM-1378 or KSM-K38.

BEST MODE FOR CARRYING OUT THE INVENTION

The term “highly productive mutant α-amylase” as used herein means an α-amylase whose yield is increased, upon production of it by cultivating a recombinant microorganism, by at least 5%, preferably at least 10%, more preferably at least 20% compared with that before mutation.

The mutant α-amylase of the present invention is constructed so that out of amino acids constituting the α-amylase, the amino acid residues taking part in the productivity are substituted with another amino acid residues or deleted. Examples of the α-amylase usable here include liquefying α-amylases derived from Bacillus. amyloliquefaciens or Bacillus. licheniformis and liquefying alkaline α-amylases derived from alkaliphilic microorganisms belonging to the Bacillus sp., of which α-amylases having an amino acid sequence represented by SEQ ID No. 2 or SEQ ID No. 4 and α-amylases having at least 60% homology to the above-described amino acid sequence are preferred.

Examples of the α-amylase having the amino acid sequence represented by SEQ ID NO. 2, or α-amylase having at least 60% homology thereto include liquefying alkaline α-amylases derived from the strain Bacillus sp. KSM-AF1378 (FERM BP-3048) (Japanese Patent Application. Laid-Open No. Hei 8-336392) and improved enzymes of the above-described one in heat resistance and oxidant resistance which are constructed by protein engineering technique (WO98/44126).

Examples of the α-amylase having the amino acid sequence represented by SEQ ID No. 4 or having at least 60% homology thereto include liquefying alkaline α-amylases derived from the strain Bacillus sp. KSM-K38 (FERM BP-6946) and improved enzymes of the above-described one in heat resistance which are constructed by protein engineering technique (Japanese Patent Application No. Hei 11-163569).

The homology of an amino acid sequence is calculated by Lipman-Pearson method (Science, 227, 1435(1985)).

The mutant α-amylase of the present invention can be obtained first by cloning, from a microorganism producing an α-amylase, a gene encoding the α-amylase. For this purpose, ordinarily employed gene recombinant technique, for example, the method as described in Japanese Patent Application Laid-Open No. Hei 8-336392 may be employed. Examples of the gene usable here include that represented by from the above-described ones and having improved heat resistance and oxidant resistance are also usable.

For mutation of the gene thus obtained by cloning, any site-directed mutagenesis ordinarily employed can be adopted. For example, mutation can be conducted using a “Site-Directed Mutagenesis System Mutan-Super Express Km” kit (product of Takara Shuzo Co., Ltd.).

Mutation for obtaining highly productive α-amylases of the invention can be attained, for example, by substitution or deletion, in an α-amylase having an amino acid sequence represented by SEQ ID No. 2 or having at least 60% homology thereto, of at least one amino acid residue corresponding to any one of Pro₁₈, Gln₈₆, Glu₁₃₀, Asn₁₅₄, Arg₁₇₁, Ala₁₈₆, Glu₂₁₂, Val₂₂₂, Tyr₂₄₃, Pro₂₆₀, Lys₂₆₉, Glu₂₇₆, Asn₂₇₇, Arg₃₁₀, Glu₃₆₀, Gln₃₉₁, Trp₄₃₉, Lys₄₄₄, Asn₄₇₁ and Gly₄₇₆ of the amino acid sequence; or by substitution or deletion, in another α-amylase having an amino acid sequence represented by SEQ ID No.4 or having at least 60% homology thereto, of at least one amino acid residue corresponding to any one of Asp₁₂₈, Gly₁₄₀, Ser₁₄₄, Arg₁₆₈, Asn₁₈₁, Glu₂₀₇, Phe₂₇₂, Ser₃₇₅, Trp₄₃₄ and Glu₄₆₆ of the amino acid sequence. Preferred mutations include, in the amino acid sequence of SEQ ID No. 2 substitution of the amino acid residue corresponding to Pro₁₈ with Ser or Thr, the amino acid residue corresponding to Gln₈₆ with Glu, the amino acid residue corresponding to Glu₁₃₀ with Val or Gln, the amino acid residue corresponding to Asn₁₅₄ with Asp, the amino acid residue corresponding to Arg₁₇₁ with Cys or Gln, the amino acid residue corresponding to Ala₁₈₆ with Val or Asn, the amino acid residue corresponding to Glu₂₁₂ with Asp, the amino acid residue corresponding to Val₂₂₂ with Gln, the amino acid residue corresponding to Tyr₂₄₃ with Cys or Ser, the amino acid residue corresponding to Pro₂₆₀ with Glu, the amino acid residue corresponding to Lys₂₆₉, with Gln, the amino acid residue corresponding to Glu₂₇₆ with His, the amino acid residue corresponding to Asn₂₇₇ with Ser or Phe, the amino acid residue corresponding to Arg₃₁₀ with Ala, the amino acid residue corresponding to Glu₃₆₀ with Gln, the amino acid residue corresponding to Gln₃₉₁ with Glu, the amino acid residue corresponding to Trp₄₃₉ with Arg, the amino acid residue corresponding to Lys₄₄₄ with Arg, the amino acid residue corresponding to Asn₄₇₁, with Asp or Glu, or the amino acid residue corresponding to Gly₄₇₆ with Asp;

or substitution, in the amino acid sequence of SEQ ID No. 4, of the amino acid residue corresponding to Asp₁₂₈ with Val or Gln, the amino acid residue corresponding to Gly₁₄₀ with Ser, the amino acid residue corresponding to Ser₁₄₄ with Pro, the amino acid residue corresponding to Arg₁₆₈ with Gln, the amino acid residue corresponding to Gln₁₈₁, with Val, the amino acid residue corresponding to Glu₂₇₀ with Asp, the amino acid residue corresponding to Phe₂₇₂ with Ser, the amino acid residue corresponding to Ser₃₇₅ with Pro, the amino acid residue corresponding to Trp₄₃₄ with Arg or the amino acid residue corresponding to Glu₄₆₆ with Asp.

Among the mutations of the amino acid sequence of SEQ ID No. 2, those by substitution of the amino acid residue corresponding to Gln₈₆ with Glu, the amino acid residue corresponding to Glu₁₃₀ with Val or Gln, the amino acid residue corresponding to Ala₁₈₆ with Asn, the amino acid residue corresponding to Tyr₂₄₃ with Ser, the amino acid residue corresponding to Pro₂₆₀ with Glu, the amino acid residue corresponding to Lys₂₆₉ with Gln, the amino acid residue corresponding to Asn₂₇₇ with Phe and the amino acid residue corresponding to Asn₄₇₁ with Asp or Gln can bring about improvement in solubility of the α-amylase in a culture medium or desalted and concentrated solution thereof. More specifically, the above-described mutations make it possible to improve the residual activity of the αamylase in the supernatant after storage at 4° C. for one week in a desalted and concentrated solution by at least 5%, especially 10% compared with the activity before mutation. Accordingly, in the case of the mutant α-amylases of the present invention obtained by such amino acid mutations, a fermented concentrate solution of a high concentration is available at a high yield and enzyme formulation treatment such as ultrafiltration after fermentation production can be conducted efficiently.

A combination of two or more substitutions or deletions of various amino acid residues is also effective for such amino acid mutations. It is also possible to use the above-exemplified mutation in combination with a mutation for improving enzymatic properties, for example, in an α-amylase having an amino acid sequence represented SEQ ID No. 2 or having at least 60% homology thereto, a mutation for improving heat resistance by deleting amino acid residues corresponding to Arg₁₈₁ and Gly₁₈₂, a mutation for improving oxidant resistance by substituting the amino acid residue corresponding to Met₂₂₂ with Thr or a mutation for improving solubility by substituting the amino acid residue corresponding Lys₄₈₄ with Gln; or in an α-amylase having an amino acid sequence represented by SEQ ID No. 4 or having at least 60% homology thereto, a mutation for further reinforcing oxidant resistance by substituting the amino acid residue corresponding to Met₁₀₇ with Leu or a mutation for heightening detergency of a laundry detergent by substituting the amino acid residue corresponding Glu₁₈₈ with Ile.

A mutant α-amylase is available at a high yield by appropriately combining a mutant α-amylase structural gene with a control gene and a proper plasmid vector, thereby constructing a plasmid for the production of the α-amylase, introducing the resulting plasmid into a microorganism such as Bacillus subtilis or Escherichia coli, preferably, Bacillus subtilis and cultivating the resulting recombinant microorganism.

The mutant α-amylase thus obtained has improved productivity by about 10 to 500% as shown later in Examples while maintaining biochemical properties as an enzyme, thus having excellent properties. By the above-described mutation of the amino acid residues of liquefying alkaline α-amylases having heat resistance, chelating agent resistance, oxidant resistance and high solubility, it is therefore possible to create useful enzymes having drastically improved productivity in a recombinant microorganism without losing the above-described original properties.

The detergent compositions of the present invention may contain, in addition to the α-amylase of the invention, one or more than one enzymes selected from debranching enzymes (such as pullulanase, isoamylase and neopullulanase), α-glucosidase, glucoamylase, protease, cellulase, lypase, pectinase, protopectinase, pectate lyase, peroxidase, laccase and catalase.

The detergent composition may contain, in addition, components ordinarily added to a detergent, for example, surfactants such as anionic surfactants, amphoteric surfactants, nonionic surfactants and cationic surfactants, chelating agents, alkali agents, inorganic salts, anti-redeposition agents, chlorine scavengers, reducing agents, bleaching agents, fluorescent dye solubilizing agents, perfumes, anti-caking agents, enzyme activating agents, antioxidants, antiseptics, blueing agents, bleach activating agents, enzyme stabilizing agents and regulator.

The detergent compositions of the invention can be produced in a manner known per se in the art from a combination of the highly productive α-amylase available by the above-described method and the above-described known detergent components. The form of the detergent can be selected according to the using purpose and examples include liquid, powder and granule. The detergent compositions of the present invention are suited as laundry detergents, bleaching detergents, detergents for automatic dish washer, pipe cleaners, and artificial tooth cleaners, of which they are especially suited as laundry detergents, bleaching detergents and detergents for automatic dish washer.

The highly productive mutant α-amylases of the invention are also usable as starch liquefying saccharifying compositions. Moreover, these mutant α-amylases, after addition thereto of one or more than one enzymes selected from glucoamylase, maltase, pullulanase, isoamylase and neopullulanase, can be allowed to act on starches.

Furthermore, the mutant α-amylases of the present invention are usable as a desizing composition of fibers and an enzyme such as pullulanase, isoamylase or neopullulanase can be incorporated in the composition.

EXAMPLES

Measurement of Amylase Activity and Protein Content

Amylase activity and protein content of the enzymes each produced from recombinant Bacillus subtilis were measured in accordance with the below-described methods.

Amylase activity was measured by the 3,5-dinitrosalicylic acid method (DNS method). After reaction at 50° C. for 15 minutes in a reaction mixture of a 40 mM glycine-sodium hydroxide buffer (pH 10) containing soluble starch, the reducing sugar thus formed was quantitatively analyzed by the DNS method. As the titer of the enzyme, the amount of the enzyme which formed reducing sugar equivalent to 1 μmol of glucose in one minute was defined as one unit.

The protein content was determined by “Protein Assay Kit” (product of Bio-Rad Laboratories) using bovine serum albumin as standard.

Referential Example 1

Screening of Liquefying Alkaline Amylase

About 0.5 g of soil was suspended in sterilized water and the resulting suspension was heat treated at 80° C. for 15 minutes. The supernatant of the heat treated mixture was diluted with an adequate amount of sterilized water, followed by applying to an isolating agar medium (Medium A). The medium was then cultured at 30° C. for 2 days to grow colonies. The colonies which formed transparent zones in their peripheries due to starch dissolution were selected and isolated as amylase producing strains. The resulting isolated strains were inoculated in Medium B, followed by aerobic shaken culture at 30° C. for 2 days. After cultivation, the chelating agent (EDTA) resisting capacity of the supernatant obtained by centrifugation was measured and in addition, the optimum working pH was measured. Thus, strain Bacillus sp. KSM-K38 (FERM BP-6946) was obtained.

Medium A: Tryptone 1.5% Soytone 0.5% Sodium chloride 0.5% Colored starch 0.5% Agar 1.5% Na₂Co₃ 0.5% (pH 10) Medium B: Tryptone 1.5% Soytone 0.5% Sodium chloride 0.5% Soluble starch 1.0% Na₂CO₃ 0.5% (pH 10)

The mycological properties of strain KSM-K38 are shown in Table 1.

TABLE 1 Strain KSM-K38 (a) Observation under microscope Cells are rods of a size of 1.0 to 1.2 μm × 2.4 to 5.4 μm in the strain K36 and 1.0 to 1.2 μm × 1.8 to 3.8 μm in the strain K38, and form an elliptical endospore (1.0 to 1.2 μm × 1.2 to 1.4 μm) at their subterminals or center. They have flagella and are motile. Gram's staining is positive. Acid fastness: negative. (b) Growth in various culture mediums. The strains are alikaliphilic so that 0.5% sodium carbonate was added to the culture medium in the tests described hereinafter. Nutrient agar plate culture Growth of cells is good. Colony has a circular shape, with its surface being smooth and its peripheral end being smooth. The color of the colony is yellowish brown. Nutrient agar slant culture Cells can grow. Nutrient broth Cells can grow. Stab culture in nutrient-broth gelatin Growth of cells is good. Liquefaction of gelatin is not observed. Litmus milk medium No change in growth. (c) Physiological properties Nitrate reduction and denitrification Nitrate reduction: positive Denitrification: negative MR test Indeterminable because the medium is an alkaline medium. V-P test Negative Production of indole Negative Production of hydrogen sulfide Negative Hydrolysis of starch Positive Utilization of citric acid Positive in Christensen's medium but negative in Koser's medium and Simmon's medium. Utilization of inorganic nitrogen sources Nitrate is utilized but ammonium salts are not. Production of colorants Negative Urease Negative Oxidase Negative Catalase Positive Growth range Growth temperature range: 15 to 40° C., optimum growth temperature: 30° C., growth pH range: pH 9.0 to 11.0, optimum growth pH range: same Behavior on oxygen Aerobic O-F test Cells do not grow Sugar utilization L-galactose, D-xylose, L-arabinose, lactose, glycerin, melibiose, ribose, D-glucose, D-mannose, maltose, sucrose, trehalose, D-mannitol, starch, raffinose and D-fructose are utilized. Growth in a salt-containing medium Cells can grow when salt concentration is 12%, but not when salt concentration is 15%.

Referential Example 2

Cultivation of Strain KSM-K38

In the liquid medium B of Referential Example 1, the strain KSM-K38 was inoculated, followed by aerobic shaken culture at 30° C. for 2 days. The amylase activity (at pH 8.5) of each of the supernatants isolated by centrifugation was measured. As a result, the activity in 1 L of the culture medium was found to be 1177 U.

Referential Example 3

Purification of Liquefying Alkaline Amylase

Ammonium sulfate was added to the supernatant of the culture medium of the strain KSM-K38 obtained in Referential Example 2 to give 80% saturation, followed by stirring. The precipitate thus formed was collected and dissolved in a 10 mM tris-HCl buffer (pH 7.5) containing 2 mM CaCl₂ to dialyze the resulting solution against the buffer overnight. The dialysate was loaded on a DEAE-Toyopearl 650M column equilibrated with the same buffer and protein was eluted in a linear gradient of 0 to 1 M of NaCl in the same buffer. The active fraction obtained by gel filtration column chromatography after dialysis against the same buffer was dialyzed against the buffer, whereby purified enzyme exhibited a single band on polyacrylamide gel electrophoresis (gel concentration: 10%) and sodium dodecylsulfate (SDS) electrophoresis was obtained.

Referential Example 4

Enzymological Properties

The properties of the purified enzyme are as follows:

(1) Action

It acts on starch, amylose, amylopectin and α-1,4-glycoside bond which is a partially degraded product thereof to degrade them and produce, from amylose, glucose (G1), maltose (G2), maltotriose (G3), maltotetraose (G4), maltopentaose (G5), maltohexaose (G6) and maltoheptaose (G7). But it does not act on pullulan.

(2) pH Stability (Britton-Robinson Buffer)

It exhibits residual activity of 70% or more within a range of pH 6.5 to 11.0 under treating conditions at 40° C. for 30 minutes.

(3) Working Temperature Range and Optimum Working Temperature

It acts in a wide temperature range of from 20 to 80° C., with the optimum working temperature being 50 to 60° C.

(4) Temperature Stability

The temperature at which the enzyme loses its activity was examined by causing a temperature change in a 50 mM glycine-sodium hydroxide buffer (pH 10) and then, treating at each temperature for 30 minutes. The residual activity of the enzyme is 80% or more at 40° C. and about 60% even at 45° C.

(5) Molecular Weight

The molecular weight as measured by sodium-dodecylsulfate polyacrylamide gel electrophoresis is 55,000 ±5,000.

(6) Isoelectric Point

Its isoelectric point as measured by isoelectric focusing electrophoresis is about 4.2.

(7) Effects of Surfactants

It is almost free from activity inhibition (activity remaining ratio: 90% or more) even when treated at pH 10 and 30° C. for 30 minutes in a 0.1% solution of a surfactant such as sodium linear alkylbenzene sulfonate, alkyl sulfate ester sodium salt, polyoxyethylene alkylsulfate ester sodium salt, sodium α-olefin sulfonate, sodium α-sulfonated fatty acid ester, sodium alkylsulfonate, SDS, soap and softanol.

(8) Effects of Metal Salts

It was treated at pH 10 and 30° C. for 30 minutes in each of the reaction systems containing various metal salts and their effects were studied. Its activity is inhibited by 1 mM of Mn²⁺ (inhibition ratio: about 75%) and slightly inhibited by 1 mM of Sr²⁺ and Cd²⁺ (inhibition ratio: about 30%).

Example 1

Preparation of Various Recombinant Plasmids Having an α-Amylase Gene Ligated Thereto

In accordance with the method as described in WO98/44126, genes encoding a mutant α-amylase (which will hereinafter be described as “ΔRG”) having improved heat resistance and a mutant α-amylase (“ΔRG-M202T”) having improved oxidant resistance as well as improved heat resistance were constructed, respectively, by deleting Arg₁₈₁ and Gly₁₈₂ of the α-amylase (“LAMY”) which was derived from the strain Bacillus sp. KSM-AP1378 (FERM BP-3048) and had the amino acid sequence represented by SEQ ID No. 2; and by, in addition to this mutation by deletion, substituting Thr for Met₂₀₂ of the amino acid sequence represented by SEQ ID No. 2. With the genes as a template, gene fragments (about 1.5 kb) encoding these mutant α-amylases were amplified by the PCR reaction using primers LAUS (SEQ ID No. 5) and LADH (SEQ ID No. 6). After cutting of them with a restriction enzyme SalI, each of the fragments was inserted into the SalI-SmaI site of an expression vector pHSP64 (Japanese Patent Application Laid-Open No. Hei 6-217781), whereby a recombinant plasmid having a structural gene of each of the mutant α-amylases bonded thereto was constructed downstream of a strong promoter derived from an alkaline cellulase gene of the strain Bacillus sp. KSM-64 (FERM P-10482).

In the meantime, with a chromosomal DNA, which had been extracted from the cells of the strain Bacillus sp. KSM-K38 (FERM BP-6946) by the method of Saito and Miura (Biochim. Biophys. Acta, 72, 619 (1961)), as a template, PCR reaction was effected using primers K38US (SEQ ID No. 7) and K38DH (SEQ ID No. 8) shown in Table 2, whereby a structural gene fragment (about 1.5 kb) encoding an α-amylase (which will hereinafter be described as “K38AMY”) having an amino acid sequence of SEQ ID No.4 was amplified. After cutting of it with a restriction enzyme SalI, the resulting fragment was inserted into the SalI-SmaI site of an expression vector pHSP64 to construct, downstream of a strong promoter derived from an alkaline cellulase gene of the strain Bacillus sp. KSM-64 (FERM P-10482) contained in pHSP64, a recombinant plasmid having a structural gene of K38AMY bonded thereto (FIG. 1). With this recombinant plasmid as a template, PCR reaction was effected using the primers CLUBG (SEQ ID. No. 9) and K38DH (SEQ. ID. No.8) to amplify a gene fragment (about 2.1 kb) having the strong promoter and K38AMY bonded thereto.

By the recombinant PCR method as described below, a gene encoding chimeric α-amylase between K38AMY and LAMY was constructed. Described specifically, with a chromosomal DNA of the strain K.SM-K38 (FERM BP6946) as a template, PCR reaction was conducted using primers K38DH (SEQ ID No. 8) and LA-K38 (SEQ ID No. 10) shown in Table 2, whereby a fragment encoding the sequence from Gln₂₀ downstream to the C-terminal of the amino acid sequence of K38AMY represented by SEQ ID No. 4 was amplified. With the above-described recombinant plasmid containing the LAMY gene and strong promoter as a template, PCR reaction was conducted using primers CLUBG (SEQ ID No. 9) and LA-K38R (SEQ ID No. 11) shown in Table 2, whereby a gene fragment encoding from the upstream strong promoter to Gly₂₁ of the amino acid sequence of LAMY of SEQ ID No. 2 was amplified. By the second PCR reaction using the resulting two DNA fragments and primers CLUBG (SEQ ID No. 9) and K38DH (SEQ ID No. 8) shown in Table 2, the resulting two fragments having, at the end thereof, complementary sequences derived from primers LA-K38 (SEQ ID No. 10) and LA-K38R (SEQ ID No. 11) respectively were combined, whereby a gene fragment (about 2.1 kb) encoding a chimeric α-amylase (which will hereinafter be described as “LA-K38AMY”) which has, successively bonded thereto, a region encoding from His₁ to Gly₂₁ of the LAMY downstream of the strong promoter and a region encoding from Gln₂₀ to the C-terminal of the K38AMY was amplified.

By using a “Site-Directed Mutagenesis System MutanSuper Express Km” kit (product of Takara Shuzo Co., Ltd.), the below-described mutations were introduced to the K38AMY and LA-K38AMY. First, the K38AMY and LA-K38AMY gene fragments (about 2.1 kb) were inserted into the site SmaI of a plasmid vector pKF19k attached to the kit to construct a mutagenic recombinant plasmid (FIG. 2). A site-directed mutagenic oligonucleotide primer N190F (SEQ ID No. 50) shown in Table 2 was 5′-phosphorylated with T4 DNA kinase. Using this and the above-described mutagenic recombinant plasmid, mutagenesis was effected in accordance with the method of the kit and by using the reaction product, the strain Escherichia coli MV1184 (“Competent cell MV1184”, product of Takara Shuzo Co., Ltd.) was transformed. From the transformant thus obtained, a recombinant plasmid was extracted, followed by analysis of a basic sequence, whereby mutation by substitution of Phe for Asn₁₉₀ was confirmed. By repeated introduction of mutagenic reactions into the mutated gene by successively using primers A209V (SEQ ID No. 51) and QEYK (SEQ ID No. 49) in a similar manner as above, thereby substituting Asn₁₉₀ and Gln₂₀₉, each of the amino acid sequence of the K38AMY represented by SEQ ID No. 4 with Phe and Val, respectively, and the sequence from Asp₁ to Gly₁₉ of the amino acid sequence of the K38AMY represented by SEQ ID No. 4 with the sequence from His₁ to Gly₂₁ of the amino acid sequence of the LAMY represented by SEQ ID No. 2; by substituting Gln₁₆₇, Tyr₁₆₉, Asn₁₉₀ and Gln₂₀₉, each of the amino acid sequence of the K38AMY, with Glu, Lys, Phe and Val, respectively and the sequence from Asp₁ to Gly₁₉ of the amino acid sequence of the K38AMY with the sequence from His₁ to Gly₂, of the amino acid sequence of the LAMY; and substituting Gln₁₆₇ and Tyr₁₆₉, Asn₁₉₀ and Gln₂₀₉, each of the amino acid sequence of the K38AMY, with Glu, Lys, Phe and Val, respectively, genes encoding a mutant α-amylase (which will hereinafter be described as “LA-K38AMY/NFQV”) having improved heat resistance, a mutant α-amylase (“LA-K38AMY/QEYKINFQV”) having drastically improved heat resistance, and a mutant α-amylase (“QEYK/NFQV”) having improved heat resistance were constructed, respectively.

With these genes as a template, PCR reaction was conducted using primers K38US (SEQ ID No. 7) and K38DH (SEQ ID No. 8) to amplify structural gene fragments (about 1.5 kb) encoding the mutant α-amylases were amplified. They were then inserted into the SalI-SmaI site of an expression vector pHSP64 in a similar manner as above, whereby a recombinant plasmid having structural genes of these mutant α-amylases bonded each other was constructed (FIG. 1).

Example 2

Introduction of a Mutation for Improving α-Amylase Productivity

A “Site-Directed Mutagenesis System Mutan-Super Express Km” kit of Takara Shuzo Co., Ltd. was used for site-directed mutagenesis for improving amylase productivity of recombinant Bacillus subtilis. With various recombinant plasmids obtained in Example 1 as a template, PCR reactions were effected using primers CLUBG (SEQ ID No. 9) and LADH (SEQ ID No. 6) for ARG and ΔRG/M202T, while using primers CLUBG (SEQ ID No. 9) and K38DH (SEQ ID No. 8) for K38AMY, LA-K38AMY/NFQV, LA-K38AMY/QEYK/NFQV and QEYK/NFQV, whereby fragments of about 2.1 kb from the upstream strong promoter derived from the strain KSM-64 to the downstream α-amylase gene were amplified. These amplified fragments were inserted into the SmaI site of a plasmid vector pKF19k attached to the above-described kit, whereby various mutagenetic recombinant plasmids were constructed (FIG. 2).

Various oligonucleotide primers for site-directed mutagenesis shown in Table 2 (SEQ ID Nos. 12 to 51) were 5′-phosphorylated with T4DNA kinase, and by using the resultant products and the above mutagenetic recombinant plasmids, mutagenesis was conducted in accordance with the method as described in the kit. With the reaction products, the strain Escherichia coli MV1184(“Competent Cell MV1184” product of Takara Shuzo Co., Ltd.) was transformed. From the resulting transformants, a recombinant plasmid was extracted, followed by analysis of a base sequence to confirm mutation.

TABLE 2 SeQ ID Using No. Primer Base sequence (5′-3′) purpose 5 LAUS GAGTCGACCAGCACAAGCCCATCATAATGG PCR for 6 LADH TAAAGCTTCAATTTATATTGG recombi- 7 K38US GGGTCGACCAGCACAAGCCGATGGATTGAACGGTACGATG nation 8 K38DH TAAAGCTTTTGTTATTGGTTCACGTACAC 9 CLUBG CCAGATCTACTTACCATTTTAGAGTCA 10 LA-K38 ATTTGCCAAATGACGGGCAGCATTGGAATCGGTT 11 LA-K38R AACCGATTCCAATGCTGCCCGTCATTTGGCAAAT 12 P18S TTTGAATGGCATTTGTCAAATGACGGGGAACCAC Site-directed 13 Q86E ACAAGGAGTCAGTTGGAAGGTGCCGTGACATCT mutagenesis 14 E130V CGAAACCAAGTAATATCAGGT (ΔRG) 15 N154D AATACCCATTCCGATTTTAAATGGCGC 16 R171C GATTGGGATCAGTCATGYCAGCTTCAGAACAAA 17 A186V AAATTCACCGGAAAGGTATGGGACTGGGAAGTA 18 E212D TCATCCAGATGTAATCAATG 19 V222E CTTAGAAATTGGGGAGAATGGTATACAAATACA 20 Y243C GTGAAACATATTAAATGCAGCTATACGAGAGAT 21 P260E AACACCACAGGTAAAGAAATGTTTGCAGTTGCA 22 K269Q AGAATTTTGGCAAAATGACCT 23 E276H TTGCTGCAATCCATAACTATTTAAAT 24 N277S CTTGCTGCAATCGAAAGYTATTTAAATAAAACA 25 R310A GGCTATTTTGATATGGCAAATATTTTAAATGGT 26 E360Q TCTGACAAGGCAGCAAGGTTA 27 Q391E GATCCACTTCTGGAAGCACGTCAAACG 28 W439R GGGGGTAATAAAAGAATGTATGTCGGG 29 K444R ATGTATGTCGGGCGACATAAAGCTGG 30 N471D GATGGTTGGGGGGATTTCACTGTAA 31 G476D TTCACTGTAAACGATGGGGCAGTTTCG 32 K484Q GGTTTGGGTGCAGCAATAAAT 33 P18X TTTGAATGGCATTTGNNNAATGACGGGAACCAC Site-directed 34 A186X AAATTCACCGGAAAGNNNTGGGACTGGGAAGTA mutagenesis 35 Y243X GTGAAACATATTAAANNNAGCTATACGAGAGAT (for ΔRG/ 36 N277X CTTGCTGCAATCGAANNNTATTTAAATAAAACA M2027) 37 N471E GATGGTTGGGGGGAATTCACTGTAA 38 D128V CCAACGAATCGTTGGCAGGTAATTTCAGGTGCCTACACG Site-directed 39 G140S ATTGATGCGTGGACGAGTTTCGACTTTTCAGGG mutagenesis 40 S144P TTTCGACTTTCCAGGGCGTAA for 41 R168Q GGTGTTGACTGGGATCAGCAATATCAAGAAAATCATATTTTCC K38AMY) 42 N181V CATATTTTCCGCTTTGCAAATACGGTNTGGAACAGGCGAGTG 43 E207D AATATCGACTTTAGTCATCCAGATGTACAAGATGAGTTGAAGGA 44 F272S GACGTAGGTGCTCTCGAATCTTATTTAGATGAAATGAATTGGG 45 S375P CGATAACATTCCAGCTAAAAA 46 W434R GACCTGGTGGTTCCAAGAGAATGTATGTAGGACGTCAG 47 E466D AATGGCGATGGATGGGGCGATTTCTTTACGAATGGAGGATCT 48 D128X CCAACGAATCGTTGGCAGNNNATTTCAGGTGCCTACACG 49 QEYK GTTGACTGGGATGAGCGCAAACAAGAAAATCAT 50 N190F TGGATGAAGAGTTCGGTAATTATGA 51 Q209 AGTCATCCAGAGGTCGTAGATGAGTTGAAGGAT The “N” in the base sequence means a mixed base of A, T, G and C, while “Y” means a mixed base of T and C.

By inserting an expression promoter region and the mutant α-amylase gene portion into the SmaI site of pKF19k again in a similar manner as the above, the mutation-introduced gene became a template plasmid upon introduction of another mutation. Another mutation was thus introduced in a similar manner to the above-described method.

With these mutated recombinant plasmids thus obtained as a template, PCR reaction was conducted using primers CLUBG (SEQ ID No. 9) and LADH (SEQ ID No. 6) or primers CLUBS (SEQ ID No. 9) and K38DH (SEQ ID No. 8) to amplify the mutated gene fragments. After they were cut with SalI, they were inserted into the site of SalI-SmaI site of an expression vector pHSP64, whereby various plasmids for producing mutant α-amylases were constructed (FIG. 1).

Example 3

Production of Mutant α-Amylases

The various plasmids for producing mutant α-amylases obtained in Example 2 were each introduced into the strain Bacillus subtilis ISW1214 (leuA metB5 hsdM1) in accordance with the protoplast method. The recombinant Bacillus subtilis thus obtained was cultivated at 30° C. for 4 days in a liquid medium (corn steep liquor, 4%; tryptose, 1%; meet extract, 1%, monopotassium phosphate, 0.1%, magnesium sulfate, 0.01%, maltose, 2%, calcium chloride, 0.1%, tetracycline, 15 μg/mL). The activity of each of the various mutant α-amylases was measured using the supernatant of the culture medium.

Example 4

Evaluation of Amylase Productivity-1

Each of an enzyme having Pro₁₈ of ΔRG substituted with Ser (which will hereinafter be abbreviated as “P18S/ΔRG”), an enzyme having Gln₈₆ substituted with Glu (“Q86E/ΔRG”), an enzyme having Glu₁₃₀ substituted with Val (“E130V/ΔRG”), an enzyme having Asn₁₅₄ substituted with Asp (“N154D/ΔRG”), an enzyme having Arg₁₇₁ substituted with Cys (“R171C/ΔRG”), an enzyme having Ala₁₈₆ substituted with Val (“A186V/ΔRG”), an enzyme having Glu₂₁₂ substituted with Asp (“E212D/ΔRG”), an enzyme having Val₂₂₂ substituted with Glu (“V222E/ΔRG”), an enzyme having Tyr₂₄₃ substituted with Cys (“Y243C/ΔRG”), an enzyme having Pro₂₆₀ substituted with Glu (“P260E/ΔRG”), an enzyme having Lys₂₆₉ substituted with Gln (“K269E/ΔRG”), an enzyme having Glu₂₇₆ substituted with His (“E276H/ΔRG”), an enzyme having Asn₂₇₇ substituted with Ser (“N277S/ΔRG”), an enzyme having Arg₃₁₀ substituted with Ala (“R310A/ΔRG”), an enzyme having Glu₃₆₀ substituted with Gln (“E360Q/ΔRG”), an enzyme having Gln₃₉₁ substituted with Glu (“Q391E/ΔRG”), an enzyme having Trp₄₃₉ substituted with Arg (“W439R/ΔRG”), an enzyme having Lys₄₄₄ substituted with Arg (“K444R/ΔRG”), an enzyme having Asn₄₇₁ substituted with Asp (“N471D/ΔRG”), and an enzyme having Gly₄₇₆ substituted with Asp (“G476D/ΔRG”) was assayed for amylase productivity. As a control, ΔRG was employed. A relative value (%) of amylase productivity was determined from the amylase productivity of ΔRG set at 100%. The results are shown in Table 3.

TABLE 3 Relative amylase Enzyme productivity (%) ΔRG 100 P18S/ΔRG 277 Q86E/ΔRG 119 E130V/ΔRG 362 N154D/ΔRG 146 R171C/ΔRG 235 A186V/ΔRG 485 E212D/ΔRG 327 V222E/ΔRG 135 Y243C/ΔRG 350 P260E/ΔRG 142 K269Q/ΔRG 142 E276H/ΔRG 231 N277S/ΔRG 312 R310A/ΔRG 208 E360Q/ΔRG 162 Q391E/ΔRG 127 W439R/ΔRG 312 K444R/ΔRG 112 N471D/ΔRG 292 G476D/ΔRG 296

Any one of the mutant enzymes exhibited higher amylase productivity than ΔRG, indicating that mutation heightened productivity of α-amylase in recombinant Bacillus subtilis. In particular, the productivity of each of E130V/ΔRG, A186V/ΔRG, E212D/ΔRG, Y243C/ΔRG, N277S/ΔRG and W439R/ΔRG was found to be at least 3 times greater than that of ΔRG and above all, A186V/ΔRG exhibited eminently high productivity of almost 5 times greater than that of ΔRG.

Example 5

Evaluation of Amylase Productivity-2

In a similar manner to the methods described in Examples 1, 2 and 3, each of an enzyme having Pro₁₈ of ΔRG/MT substituted with Thr (which will hereinafter be abbreviated as “P18T/ΔRG/MT”), an enzyme having Gln₈₆ substituted with Glu (“Q86E/ΔRG/MT”), an enzyme having Glu₁₃₀ substituted with Val (“E130V/ΔRG/MT”), an enzyme having Ala₁₈₆ substituted with Asn (“A186N/ΔRG/MT”), an enzyme having Tyr₂₄₃ substituted with Ser (“Y243S/ΔRG/MT”), an enzyme having Asn₂₇₇ substituted with Phe (“N277F/ΔRG/MT”), and an enzyme having Asn₄₇₁ substituted with Glu (“N471E/ΔRG/MT”) was assayed for amylase productivity. As a control, ΔRG/MT was employed. The results are shown in Table 4.

TABLE 4 Relative amylase Enzyme productivity (%) ΔRG/MT 100 P18T/ΔRG/MT 200 Q86E/ΔRG/MT 144 E130V/ΔRG/MT 344 A186N/ΔRG/MT 344 Y243S/ΔRG/MT 189 N277F/ΔRG/MT 256 N471E/ΔRG/MT 211

It was recognized that any one of the above-described mutant enzymes exhibited high amylase productivity compared with ΔRG/MT, and in particular, the productivity of each of E130V/ΔRG/MT and A186N/ΔRG/MT was at least 3 times greater than that of ΔRG/MT.

Example 6

Evaluation of Amylase Productivity-3

In accordance with the methods employed in Examples 1, 2 and 3, each of an enzyme having Asp₁₂₈ of K38AMY substituted with Val (which will hereinafter be abbreviated as “D128V”), an enzyme having Gly₁₄₀ substituted with Ser (“G140S”), an enzyme having Ser₁₄₄ substituted with Pro (“S144P”), an enzyme having Arg₁₆₈ substituted with Gln (“R168Q”), an enzyme having Asn₁₈₁ substituted with Val (“N181V”), an enzyme having Glu₂₀₇ substituted with Asp (“E207D”), an enzyme having Phe₂₇₂ substituted with Ser (“F272S”), an enzyme having Ser₃₇₅ substituted with Pro (“S375P”), an enzyme having Trp₄₃₄ substituted with Arg (“W434R”), and an enzyme having Glu₄₆₆ substituted with Asp (“E466D”) was assayed for amylase productivity. As a control, K38AMY was employed. The results are shown in Table 5.

TABLE 5 Relative amylase Enzyme productivity (%) K38AMY 100 D128V 325 G140S 209 S144P 197 R168Q 264 N181V 207 E207D 109 F272S 175 S375P 115 W434R 124 E466D 212

It was recognized that compared with the wild type K38AMY, any one of the mutant enzymes exhibited high amylase productivity and in particular, D128V exhibited high productivity at least 3 times greater than that of K38AMY.

Example 7

Evaluation of Amylase Productivity-4

A mutant enzyme S144P/N181V (which will hereinafter be abbreviated as “SPNV”) having, among the mutants shown in Example 6, S144P and N181V in combination was assayed for amylase productivity in accordance with the method as described in Example 3. As a control, K38AMY, S144P and N181V were employed. The results are shown in Table 6.

TABLE 6 Relative amylase Enzyme productivity (%) K38AMY 100 S144P 197 N181V 207 SPNV 257

As a result, as shown in Table 6, a further improvement in amylase productivity was brought about by combined use.

Example 8

Evaluation of Amylase Productivity-5

In accordance with the methods as described in Examples 1, 2 and 3, each of an enzyme obtained by substituting Arg₁₆₈ of the gene of a heat-resistance improved enzyme LA-K38AMY/NFQV with Gln (which will hereinafter be abbreviated as “R168Q/LA-K38AMY/NFQV”), an enzyme obtained by substituting Glu₄₆₆ of the above-described gene with Asp (“E466D/LA-K38AMY/NFQV”), and an enzyme having double mutations of Example 6 introduced into the gene (“SPNV/LA-K38AMY/NFQV”) was assayed for amylase productivity. As a control, LA-K38AMY/NFQV was employed. The results are shown in Table 7.

TABLE 7 Relative amylase Enzyme productivity (%) LA-K38AMY/NFQV 100 R168Q/LA-K38AMY/NFQV 304 E466D/LA-K38AMY/NFQV 264 SPNV/LA-K38AMY/NFQV 154

As a result, it was recognized that any one of the mutant enzymes obtained in this Example exhibited high amylase productivity at least about 1.5 times greater than that of LA-K38AMY/NFQV and in particular, R168Q/LA-K38AMY/NFQV exhibited about 3 times greater productivity.

Example 9

Evaluation of Amylase Productivity-6

In accordance with the methods as described in Examples 1, 2 and 3, each of an enzyme obtained by substituting Asp₁₂₈ of the gene of a heat-resistance improved enzyme LA-K38AMY/QEYK/NFQV with Val (which will hereinafter be abbreviated as “D128V/LA-K38AMY/QEYK/NFQV”) and an enzyme having double mutations of Example 6 introduced into the gene (“SPNV/LA-K38AMY/QEYK/NFQV”) was assayed for amylase productivity. As a control, LA-K38AMY/QEYK/NFQV was employed. The results are shown in Table 8.

TABLE 8 Relative amylase Enzyme productivity (%) LA-K38AMY/QEYK/NFQV 100 D128V/LA-K38AMY/QEYK/NFQV 602 SPNV/LA-K38AMY/QEYK/NFQV 427

As a result, it was recognized that any one of the mutant enzymes obtained in this Example exhibited markedly high amylase productivity compared with LA-K38AMY/QEYK/NFQV and in particular, D128V/LA-K38AMY/QEYK/NFQV exhibited drastic increase (about 6 times) in productivity.

Example 10

Evaluation of Amylase Productivity-7

Into D128V/LA-K38AMY/QEYK/NFQV which was recognized to show a drastic increase in productivity among the mutant enzymes shown in Example 9, a mutation for heightening oxidant resistance by substituting Met₁₀₇ with Leu (this mutation will hereinafter be abbreviated as “M107L”) was introduced in accordance with the methods as described in Examples 1 and 2 (“ML/DV/LA-K38AMY/QEYK/NFQV”).

Then, the gene of the mutant enzyme ML/DV/LA-K38AMY/QEYK/NFQV was assayed for amylase productivity in accordance with the method of Example 4. As a control, D128V/LA-K38AMY/QEYK/NFQV was employed. The results are shown in Table 9.

TABLE 9 Relative amylase Enzyme productivity (%) D128V/LA-K38AMY/QEYK/NFQV 100 M107L/D128V/LA- 115 K38AMY/QEYK/NFQV

The relative amylase productivity of the mutant enzyme ML/DV/LA-K38AMY/QEYK/NFQV was 115%, indicating that introduction of M107L mutation for reinforcing oxidant resistance did not adversely affect high productivity of amylase in recombinant Bacillus subtilis.

Example 11

Evaluation of Amylase Productivity-8

In accordance with the methods as described in Examples 1, 2 and 3, an enzyme obtained by substituting AsP₁₂₈ of the gene of heat-resistance-improved enzyme QEYK/NFQV with Gln (the resultant enzyme will hereinafter be abbreviated as “D128Q/QEYK/NFQV”) was assayed for amylase productivity. As a control, QEYK/NFQV was employed. The results are shown in Table 10.

TABLE 10 Relative amylase Enzyme productivity (%) QEYK/NFQV 100 D128Q/QEYK/NFQV 247

It was recognized that the mutant enzyme exhibited productivity of at least 2 times greater than that of QEYK/NFQV.

Example 12

Solubility Assay

After storage of each of the mutant enzyme preparations as shown in Table 11 at 4° C. for 1 week, the precipitate formed by centrifugation (13000 rpm, 10 minutes, 4° C.) was separated. The precipitate was suspended in the same volume, as that before centrifugation, of a Tris-HCl buffer (pH 7.0) containing of 2 mM CaCl₂. The resulting suspension was diluted about 500-folds with the same buffer to dissolve the former in the latter and enzymatic activity in the resulting solution was measured. The supernatant was diluted in a similar manner and enzymatic activity in it was also measured. Solubility of each of the mutant enzymes was evaluated by comparing the enzymatic activity in each of the precipitate solution and supernatant with that of the preparation before storage at 4° C. The results are shown collectively in Table 11.

TABLE 11 Residual activity (%) after storage at 4° C. Enzyme Supernatant Precipitate ΔRG 55 40 ΔRG Gln86 → Glu 83 11 ΔRG Pro260 → Glu 70 18 ΔRG Lys269 → Gln 74 27 ΔRG Asn471 → Asp 74 23 ΔRG Lys484 → Gln 71 24

As a result, when an improved α-amylase (ΔRG) having heat resistance improved by deleting Arg₁₈₁ and Gly₁₈₂ was stored at 4° C. for one week, precipitation of the enzyme was recognized and only about half of the activity remained in the supernatant. On the other hand, the mutant enzymes obtained by introducing a further mutation in ΔRG-LAMY showed a high activity residual ratio in the supernatant, indicating an improvement in solubility by mutation. In particular, the enzyme having Gln₈₆ substituted with Glu showed the highest enzyme solubility and 80% of the enzyme remained in the supernatant under the conditions of this Example.

Example 13

Detergent Composition for Automatic Dish Washer

A detergent composition for automatic dish washer having the composition as shown in Table 12 was prepared, followed by incorporation therein of various mutant enzymes obtained in the productivity increasing method. As a result, the highly productive mutant enzymes exhibited similar or superior detergency to the control enzyme when they were equal in activity.

TABLE 12 Composition of detergent (%) Pluronic L-61 2.2 Sodium carbonate 24.7 Sodium bicarbonate 24.7 Sodium percarbonate 10.0 No. 1 sodium silicate 12.0 Trisodium citrate 20.0 Polypropylene glycol 2.2 “Silicone KST-04” (product of Toshiba Silicone) 0.2 “Sokalan CP-45” (product of BASF) 4.0

Capability of Exploitation Industry

By using the mutant α-amylases according to the present invention, α-amylases are available at a high yield from recombinant microorganisms, making it possible to largely reduce the cost of their industrial production. The mutation for productivity increase in the present invention does not adversely affect biochemical properties of the enzymes so that highly productive liquefying alkaline α-amylases having heat resistance, chelating agent resistance and oxidant resistance and being useful as enzymes for a detergent can be produced.

51 1 1786 DNA Bacillus sp. KSM-AP1378 sig_peptide (155)..(247) 1 cagcgtgata atataaattt gaaatgaaca cctatgaaaa tatggtagcg attgcgcgac 60 gagaaaaaac ttgggagtta ggaagtgata ttaaaggatt ttttttgact tgttgtgaaa 120 acgcttgcat aaattgaagg agagggtgct tttt atg aaa ctt cat aac cgt ata 175 Met Lys Leu His Asn Arg Ile -30 -25 att agc gta cta tta aca cta ttg tta gct gta gct gtt ttg ttt cca 223 Ile Ser Val Leu Leu Thr Leu Leu Leu Ala Val Ala Val Leu Phe Pro -20 -15 -10 tat atg acg gaa cca gca caa gcc cat cat aat ggg acg aat ggg acc 271 Tyr Met Thr Glu Pro Ala Gln Ala His His Asn Gly Thr Asn Gly Thr -5 -1 1 5 atg atg cag tat ttt gaa tgg cat ttg cca aat gac ggg aac cac tgg 319 Met Met Gln Tyr Phe Glu Trp His Leu Pro Asn Asp Gly Asn His Trp 10 15 20 aac agg tta cga gat gac gca gct aac tta aag agt aaa ggg att acc 367 Asn Arg Leu Arg Asp Asp Ala Ala Asn Leu Lys Ser Lys Gly Ile Thr 25 30 35 40 gct gtt tgg att cct cct gca tgg aag ggg act tcg caa aat gat gtt 415 Ala Val Trp Ile Pro Pro Ala Trp Lys Gly Thr Ser Gln Asn Asp Val 45 50 55 ggg tat ggt gcc tat gat ttg tac gat ctt ggt gag ttt aac caa aag 463 Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu Gly Glu Phe Asn Gln Lys 60 65 70 gga acc gtc cgt aca aaa tat ggc aca agg agt cag ttg caa ggt gcc 511 Gly Thr Val Arg Thr Lys Tyr Gly Thr Arg Ser Gln Leu Gln Gly Ala 75 80 85 gtg aca tct ttg aaa aat aac ggg att caa gtt tat ggg gat gtc gtg 559 Val Thr Ser Leu Lys Asn Asn Gly Ile Gln Val Tyr Gly Asp Val Val 90 95 100 atg aat cat aaa ggt gga gca gac ggg aca gag atg gta aat gcg gtg 607 Met Asn His Lys Gly Gly Ala Asp Gly Thr Glu Met Val Asn Ala Val 105 110 115 120 gaa gtg aac cga agc aac cga aac caa gaa ata tca ggt gaa tac acc 655 Glu Val Asn Arg Ser Asn Arg Asn Gln Glu Ile Ser Gly Glu Tyr Thr 125 130 135 att gaa gca tgg acg aaa ttt gat ttc cct gga aga gga aat acc cat 703 Ile Glu Ala Trp Thr Lys Phe Asp Phe Pro Gly Arg Gly Asn Thr His 140 145 150 tcc aac ttt aaa tgg cgc tgg tat cat ttt gat ggg aca gat tgg gat 751 Ser Asn Phe Lys Trp Arg Trp Tyr His Phe Asp Gly Thr Asp Trp Asp 155 160 165 cag tca cgt cag ctt cag aac aaa ata tat aaa ttc aga ggt acc gga 799 Gln Ser Arg Gln Leu Gln Asn Lys Ile Tyr Lys Phe Arg Gly Thr Gly 170 175 180 aag gca tgg gac tgg gaa gta gat ata gag aac ggc aac tat gat tac 847 Lys Ala Trp Asp Trp Glu Val Asp Ile Glu Asn Gly Asn Tyr Asp Tyr 185 190 195 200 ctt atg tat gca gac att gat atg gat cat cca gaa gta atc aat gaa 895 Leu Met Tyr Ala Asp Ile Asp Met Asp His Pro Glu Val Ile Asn Glu 205 210 215 ctt aga aat tgg gga gtt tgg tat aca aat aca ctt aat cta gat gga 943 Leu Arg Asn Trp Gly Val Trp Tyr Thr Asn Thr Leu Asn Leu Asp Gly 220 225 230 ttt aga atc gat gct gtg aaa cat att aaa tac agc tat acg aga gat 991 Phe Arg Ile Asp Ala Val Lys His Ile Lys Tyr Ser Tyr Thr Arg Asp 235 240 245 tgg cta aca cat gtg cgt aac acc aca ggt aaa cca atg ttt gca gtt 1039 Trp Leu Thr His Val Arg Asn Thr Thr Gly Lys Pro Met Phe Ala Val 250 255 260 gca gaa ttt tgg aaa aat gac ctt gct gca atc gaa aac tat tta aat 1087 Ala Glu Phe Trp Lys Asn Asp Leu Ala Ala Ile Glu Asn Tyr Leu Asn 265 270 275 280 aaa aca agt tgg aat cac tcc gtg ttc gat gtt cct ctt cat tat aat 1135 Lys Thr Ser Trp Asn His Ser Val Phe Asp Val Pro Leu His Tyr Asn 285 290 295 ttg tac aat gca tct aat agt ggt ggc tat ttt gat atg aga aat att 1183 Leu Tyr Asn Ala Ser Asn Ser Gly Gly Tyr Phe Asp Met Arg Asn Ile 300 305 310 tta aat ggt tct gtc gta caa aaa cac cct ata cat gca gtc aca ttt 1231 Leu Asn Gly Ser Val Val Gln Lys His Pro Ile His Ala Val Thr Phe 315 320 325 gtt gat aac cat gac tct cag cca gga gaa gca ttg gaa tcc ttt gtt 1279 Val Asp Asn His Asp Ser Gln Pro Gly Glu Ala Leu Glu Ser Phe Val 330 335 340 caa tcg tgg ttc aaa cca ctg gca tat gca ttg att ctg aca agg gag 1327 Gln Ser Trp Phe Lys Pro Leu Ala Tyr Ala Leu Ile Leu Thr Arg Glu 345 350 355 360 caa ggt tac cct tcc gta ttt tac ggt gat tac tac ggt ata cca act 1375 Gln Gly Tyr Pro Ser Val Phe Tyr Gly Asp Tyr Tyr Gly Ile Pro Thr 365 370 375 cat ggt gtt cct tcg atg aaa tct aaa att gat cca ctt ctg cag gca 1423 His Gly Val Pro Ser Met Lys Ser Lys Ile Asp Pro Leu Leu Gln Ala 380 385 390 cgt caa acg tat gcc tac gga acc caa cat gat tat ttt gat cat cat 1471 Arg Gln Thr Tyr Ala Tyr Gly Thr Gln His Asp Tyr Phe Asp His His 395 400 405 gat att atc ggc tgg acg aga gaa ggg gac agc tcc cac cca aat tca 1519 Asp Ile Ile Gly Trp Thr Arg Glu Gly Asp Ser Ser His Pro Asn Ser 410 415 420 gga ctt gca act att atg tcc gat ggg cca ggg ggt aat aaa tgg atg 1567 Gly Leu Ala Thr Ile Met Ser Asp Gly Pro Gly Gly Asn Lys Trp Met 425 430 435 440 tat gtc ggg aaa cat aaa gct ggc caa gta tgg aga gat atc acc gga 1615 Tyr Val Gly Lys His Lys Ala Gly Gln Val Trp Arg Asp Ile Thr Gly 445 450 455 aat agg tct ggt acc gtc acc att aat gca gat ggt tgg ggg aat ttc 1663 Asn Arg Ser Gly Thr Val Thr Ile Asn Ala Asp Gly Trp Gly Asn Phe 460 465 470 act gta aac gga ggg gca gtt tcg gtt tgg gtg aag caa taaataagga 1712 Thr Val Asn Gly Gly Ala Val Ser Val Trp Val Lys Gln 475 480 485 acaagaggcg aaaattactt tcctacatgc agagctttcc gatcactcat acacccaata 1772 taaattggaa gctt 1786 2 516 PRT Bacillus sp. KSM-AP1378 2 Met Lys Leu His Asn Arg Ile Ile Ser Val Leu Leu Thr Leu Leu Leu -30 -25 -20 Ala Val Ala Val Leu Phe Pro Tyr Met Thr Glu Pro Ala Gln Ala His -15 -10 -5 -1 1 His Asn Gly Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp His Leu 5 10 15 Pro Asn Asp Gly Asn His Trp Asn Arg Leu Arg Asp Asp Ala Ala Asn 20 25 30 Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp Lys 35 40 45 Gly Thr Ser Gln Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp 50 55 60 65 Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 70 75 80 Arg Ser Gln Leu Gln Gly Ala Val Thr Ser Leu Lys Asn Asn Gly Ile 85 90 95 Gln Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp Gly 100 105 110 Thr Glu Met Val Asn Ala Val Glu Val Asn Arg Ser Asn Arg Asn Gln 115 120 125 Glu Ile Ser Gly Glu Tyr Thr Ile Glu Ala Trp Thr Lys Phe Asp Phe 130 135 140 145 Pro Gly Arg Gly Asn Thr His Ser Asn Phe Lys Trp Arg Trp Tyr His 150 155 160 Phe Asp Gly Thr Asp Trp Asp Gln Ser Arg Gln Leu Gln Asn Lys Ile 165 170 175 Tyr Lys Phe Arg Gly Thr Gly Lys Ala Trp Asp Trp Glu Val Asp Ile 180 185 190 Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met Asp 195 200 205 His Pro Glu Val Ile Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr Thr 210 215 220 225 Asn Thr Leu Asn Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His Ile 230 235 240 Lys Tyr Ser Tyr Thr Arg Asp Trp Leu Thr His Val Arg Asn Thr Thr 245 250 255 Gly Lys Pro Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu Ala 260 265 270 Ala Ile Glu Asn Tyr Leu Asn Lys Thr Ser Trp Asn His Ser Val Phe 275 280 285 Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Asn Ser Gly Gly 290 295 300 305 Tyr Phe Asp Met Arg Asn Ile Leu Asn Gly Ser Val Val Gln Lys His 310 315 320 Pro Ile His Ala Val Thr Phe Val Asp Asn His Asp Ser Gln Pro Gly 325 330 335 Glu Ala Leu Glu Ser Phe Val Gln Ser Trp Phe Lys Pro Leu Ala Tyr 340 345 350 Ala Leu Ile Leu Thr Arg Glu Gln Gly Tyr Pro Ser Val Phe Tyr Gly 355 360 365 Asp Tyr Tyr Gly Ile Pro Thr His Gly Val Pro Ser Met Lys Ser Lys 370 375 380 385 Ile Asp Pro Leu Leu Gln Ala Arg Gln Thr Tyr Ala Tyr Gly Thr Gln 390 395 400 His Asp Tyr Phe Asp His His Asp Ile Ile Gly Trp Thr Arg Glu Gly 405 410 415 Asp Ser Ser His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp Gly 420 425 430 Pro Gly Gly Asn Lys Trp Met Tyr Val Gly Lys His Lys Ala Gly Gln 435 440 445 Val Trp Arg Asp Ile Thr Gly Asn Arg Ser Gly Thr Val Thr Ile Asn 450 455 460 465 Ala Asp Gly Trp Gly Asn Phe Thr Val Asn Gly Gly Ala Val Ser Val 470 475 480 Trp Val Lys Gln 485 3 1753 DNA Bacillus sp. KSM-K38 sig_peptide (162)..(224) 3 gtatgcgaaa cgatgcgcaa aactgcgcaa ctactagcac tcttcaggga ctaaaccacc 60 ttttttccaa aaatgacatc atataaacaa atttgtctac caatcactat ttaaagctgt 120 ttatgatata tgtaagcgtt atcattaaaa ggaggtattt g atg aga aga tgg gta 176 Met Arg Arg Trp Val -20 gta gca atg ttg gca gtg tta ttt tta ttt cct tcg gta gta gtt gca 224 Val Ala Met Leu Ala Val Leu Phe Leu Phe Pro Ser Val Val Val Ala -15 -10 -5 -1 gat gga ttg aac ggt acg atg atg cag tat tat gag tgg cat ttg gaa 272 Asp Gly Leu Asn Gly Thr Met Met Gln Tyr Tyr Glu Trp His Leu Glu 1 5 10 15 aac gac ggg cag cat tgg aat cgg ttg cac gat gat gcc gca gct ttg 320 Asn Asp Gly Gln His Trp Asn Arg Leu His Asp Asp Ala Ala Ala Leu 20 25 30 agt gat gct ggt att aca gct att tgg att ccg cca gcc tac aaa ggt 368 Ser Asp Ala Gly Ile Thr Ala Ile Trp Ile Pro Pro Ala Tyr Lys Gly 35 40 45 aat agt cag gcg gat gtt ggg tac ggt gca tac gat ctt tat gat tta 416 Asn Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr Asp Leu 50 55 60 gga gag ttc aat caa aag ggt act gtt cga acg aaa tac gga act aag 464 Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 65 70 75 80 gca cag ctt gaa cga gct att ggg tcc ctt aaa tct aat gat atc aat 512 Ala Gln Leu Glu Arg Ala Ile Gly Ser Leu Lys Ser Asn Asp Ile Asn 85 90 95 gta tac gga gat gtc gtg atg aat cat aaa atg gga gct gat ttt acg 560 Val Tyr Gly Asp Val Val Met Asn His Lys Met Gly Ala Asp Phe Thr 100 105 110 gag gca gtg caa gct gtt caa gta aat cca acg aat cgt tgg cag gat 608 Glu Ala Val Gln Ala Val Gln Val Asn Pro Thr Asn Arg Trp Gln Asp 115 120 125 att tca ggt gcc tac acg att gat gcg tgg acg ggt ttc gac ttt tca 656 Ile Ser Gly Ala Tyr Thr Ile Asp Ala Trp Thr Gly Phe Asp Phe Ser 130 135 140 ggg cgt aac aac gcc tat tca gat ttt aag tgg aga tgg ttc cat ttt 704 Gly Arg Asn Asn Ala Tyr Ser Asp Phe Lys Trp Arg Trp Phe His Phe 145 150 155 160 aat ggt gtt gac tgg gat cag cgc tat caa gaa aat cat att ttc cgc 752 Asn Gly Val Asp Trp Asp Gln Arg Tyr Gln Glu Asn His Ile Phe Arg 165 170 175 ttt gca aat acg aac tgg aac tgg cga gtg gat gaa gag aac ggt aat 800 Phe Ala Asn Thr Asn Trp Asn Trp Arg Val Asp Glu Glu Asn Gly Asn 180 185 190 tat gat tac ctg tta gga tcg aat atc gac ttt agt cat cca gaa gta 848 Tyr Asp Tyr Leu Leu Gly Ser Asn Ile Asp Phe Ser His Pro Glu Val 195 200 205 caa gat gag ttg aag gat tgg ggt agc tgg ttt acc gat gag tta gat 896 Gln Asp Glu Leu Lys Asp Trp Gly Ser Trp Phe Thr Asp Glu Leu Asp 210 215 220 ttg gat ggt tat cgt tta gat gct att aaa cat att cca ttc tgg tat 944 Leu Asp Gly Tyr Arg Leu Asp Ala Ile Lys His Ile Pro Phe Trp Tyr 225 230 235 240 aca tct gat tgg gtt cgg cat cag cgc aac gaa gca gat caa gat tta 992 Thr Ser Asp Trp Val Arg His Gln Arg Asn Glu Ala Asp Gln Asp Leu 245 250 255 ttt gtc gta ggg gaa tat tgg aag gat gac gta ggt gct ctc gaa ttt 1040 Phe Val Val Gly Glu Tyr Trp Lys Asp Asp Val Gly Ala Leu Glu Phe 260 265 270 tat tta gat gaa atg aat tgg gag atg tct cta ttc gat gtt cca ctt 1088 Tyr Leu Asp Glu Met Asn Trp Glu Met Ser Leu Phe Asp Val Pro Leu 275 280 285 aat tat aat ttt tac cgg gct tca caa caa ggt gga agc tat gat atg 1136 Asn Tyr Asn Phe Tyr Arg Ala Ser Gln Gln Gly Gly Ser Tyr Asp Met 290 295 300 cgt aat att tta cga gga tct tta gta gaa gcg cat ccg atg cat gca 1184 Arg Asn Ile Leu Arg Gly Ser Leu Val Glu Ala His Pro Met His Ala 305 310 315 320 gtt acg ttt gtt gat aat cat gat act cag cca ggg gag tca tta gag 1232 Val Thr Phe Val Asp Asn His Asp Thr Gln Pro Gly Glu Ser Leu Glu 325 330 335 tca tgg gtt gct gat tgg ttt aag cca ctt gct tat gcg aca att ttg 1280 Ser Trp Val Ala Asp Trp Phe Lys Pro Leu Ala Tyr Ala Thr Ile Leu 340 345 350 acg cgt gaa ggt ggt tat cca aat gta ttt tac ggt gat tac tat ggg 1328 Thr Arg Glu Gly Gly Tyr Pro Asn Val Phe Tyr Gly Asp Tyr Tyr Gly 355 360 365 att cct aac gat aac att tca gct aaa aaa gat atg att gat gag ctg 1376 Ile Pro Asn Asp Asn Ile Ser Ala Lys Lys Asp Met Ile Asp Glu Leu 370 375 380 ctt gat gca cgt caa aat tac gca tat ggc acg cag cat gac tat ttt 1424 Leu Asp Ala Arg Gln Asn Tyr Ala Tyr Gly Thr Gln His Asp Tyr Phe 385 390 395 400 gat cat tgg gat gtt gta gga tgg act agg gaa gga tct tcc tcc aga 1472 Asp His Trp Asp Val Val Gly Trp Thr Arg Glu Gly Ser Ser Ser Arg 405 410 415 cct aat tca ggc ctt gcg act att atg tcg aat gga cct ggt ggt tcc 1520 Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asn Gly Pro Gly Gly Ser 420 425 430 aag tgg atg tat gta gga cgt cag aat gca gga caa aca tgg aca gat 1568 Lys Trp Met Tyr Val Gly Arg Gln Asn Ala Gly Gln Thr Trp Thr Asp 435 440 445 tta act ggt aat aac gga gcg tcc gtt aca att aat ggc gat gga tgg 1616 Leu Thr Gly Asn Asn Gly Ala Ser Val Thr Ile Asn Gly Asp Gly Trp 450 455 460 ggc gaa ttc ttt acg aat gga gga tct gta tcc gtg tac gtg aac caa 1664 Gly Glu Phe Phe Thr Asn Gly Gly Ser Val Ser Val Tyr Val Asn Gln 465 470 475 480 taacaaaaag ccttgagaag ggattcctcc ctaactcaag gctttcttta tgtcgcttag 1724 cttaacgctt ctacgacttt gaagcttta 1753 4 501 PRT Bacillus sp. KSM-K38 4 Met Arg Arg Trp Val Val Ala Met Leu Ala Val Leu Phe Leu Phe Pro -20 -15 -10 Ser Val Val Val Ala Asp Gly Leu Asn Gly Thr Met Met Gln Tyr Tyr -5 -1 1 5 10 Glu Trp His Leu Glu Asn Asp Gly Gln His Trp Asn Arg Leu His Asp 15 20 25 Asp Ala Ala Ala Leu Ser Asp Ala Gly Ile Thr Ala Ile Trp Ile Pro 30 35 40 Pro Ala Tyr Lys Gly Asn Ser Gln Ala Asp Val Gly Tyr Gly Ala Tyr 45 50 55 Asp Leu Tyr Asp Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr 60 65 70 75 Lys Tyr Gly Thr Lys Ala Gln Leu Glu Arg Ala Ile Gly Ser Leu Lys 80 85 90 Ser Asn Asp Ile Asn Val Tyr Gly Asp Val Val Met Asn His Lys Met 95 100 105 Gly Ala Asp Phe Thr Glu Ala Val Gln Ala Val Gln Val Asn Pro Thr 110 115 120 Asn Arg Trp Gln Asp Ile Ser Gly Ala Tyr Thr Ile Asp Ala Trp Thr 125 130 135 Gly Phe Asp Phe Ser Gly Arg Asn Asn Ala Tyr Ser Asp Phe Lys Trp 140 145 150 155 Arg Trp Phe His Phe Asn Gly Val Asp Trp Asp Gln Arg Tyr Gln Glu 160 165 170 Asn His Ile Phe Arg Phe Ala Asn Thr Asn Trp Asn Trp Arg Val Asp 175 180 185 Glu Glu Asn Gly Asn Tyr Asp Tyr Leu Leu Gly Ser Asn Ile Asp Phe 190 195 200 Ser His Pro Glu Val Gln Asp Glu Leu Lys Asp Trp Gly Ser Trp Phe 205 210 215 Thr Asp Glu Leu Asp Leu Asp Gly Tyr Arg Leu Asp Ala Ile Lys His 220 225 230 235 Ile Pro Phe Trp Tyr Thr Ser Asp Trp Val Arg His Gln Arg Asn Glu 240 245 250 Ala Asp Gln Asp Leu Phe Val Val Gly Glu Tyr Trp Lys Asp Asp Val 255 260 265 Gly Ala Leu Glu Phe Tyr Leu Asp Glu Met Asn Trp Glu Met Ser Leu 270 275 280 Phe Asp Val Pro Leu Asn Tyr Asn Phe Tyr Arg Ala Ser Gln Gln Gly 285 290 295 Gly Ser Tyr Asp Met Arg Asn Ile Leu Arg Gly Ser Leu Val Glu Ala 300 305 310 315 His Pro Met His Ala Val Thr Phe Val Asp Asn His Asp Thr Gln Pro 320 325 330 Gly Glu Ser Leu Glu Ser Trp Val Ala Asp Trp Phe Lys Pro Leu Ala 335 340 345 Tyr Ala Thr Ile Leu Thr Arg Glu Gly Gly Tyr Pro Asn Val Phe Tyr 350 355 360 Gly Asp Tyr Tyr Gly Ile Pro Asn Asp Asn Ile Ser Ala Lys Lys Asp 365 370 375 Met Ile Asp Glu Leu Leu Asp Ala Arg Gln Asn Tyr Ala Tyr Gly Thr 380 385 390 395 Gln His Asp Tyr Phe Asp His Trp Asp Val Val Gly Trp Thr Arg Glu 400 405 410 Gly Ser Ser Ser Arg Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asn 415 420 425 Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Arg Gln Asn Ala Gly 430 435 440 Gln Thr Trp Thr Asp Leu Thr Gly Asn Asn Gly Ala Ser Val Thr Ile 445 450 455 Asn Gly Asp Gly Trp Gly Glu Phe Phe Thr Asn Gly Gly Ser Val Ser 460 465 470 475 Val Tyr Val Asn Gln 480 5 30 DNA Artificial Sequence Synthetic DNA 5 gagtcgacca gcacaagccc atcataatgg 30 6 21 DNA Artificial Sequence Synthetic DNA 6 taaagcttca atttatattg g 21 7 40 DNA Artificial Sequence Synthetic DNA 7 gggtcgacca gcacaagccg atggattgaa cggtacgatg 40 8 29 DNA Artificial Sequence Synthetic DNA 8 taaagctttt gttattggtt cacgtacac 29 9 27 DNA Artificial Sequence Synthetic DNA 9 ccagatctac ttaccatttt agagtca 27 10 34 DNA Artificial Sequence Synthetic DNA 10 atttgccaaa tgacgggcag cattggaatc ggtt 34 11 34 DNA Artificial Sequence Synthetic DNA 11 aaccgattcc aatgctgccc gtcatttggc aaat 34 12 34 DNA Artificial Sequence Synthetic DNA 12 tttgaatggc atttgtcaaa tgacggggaa ccac 34 13 33 DNA Artificial Sequence Synthetic DNA 13 acaaggagtc agttggaagg tgccgtgaca tct 33 14 21 DNA Artificial Sequence Synthetic DNA 14 cgaaaccaag taatatcagg t 21 15 27 DNA Artificial Sequence Synthetic DNA 15 aatacccatt ccgattttaa atggcgc 27 16 33 DNA Artificial Sequence Synthetic DNA 16 gattgggatc agtcatgyca gcttcagaac aaa 33 17 33 DNA Artificial Sequence Synthetic DNA 17 aaattcaccg gaaaggtatg ggactgggaa gta 33 18 20 DNA Artificial Sequence Synthetic DNA 18 tcatccagat gtaatcaatg 20 19 33 DNA Artificial Sequence Synthetic DNA 19 cttagaaatt ggggagaatg gtatacaaat aca 33 20 33 DNA Artificial Sequence Synthetic DNA 20 gtgaaacata ttaaatgcag ctatacgaga gat 33 21 33 DNA Artificial Sequence Synthetic DNA 21 aacaccacag gtaaagaaat gtttgcagtt gca 33 22 21 DNA Artificial Sequence Synthetic DNA 22 agaattttgg caaaatgacc t 21 23 26 DNA Artificial Sequence Synthetic DNA 23 ttgctgcaat ccataactat ttaaat 26 24 33 DNA Artificial Sequence Synthetic DNA 24 cttgctgcaa tcgaaagyta tttaaataaa aca 33 25 33 DNA Artificial Sequence Synthetic DNA 25 ggctattttg atatggcaaa tattttaaat ggt 33 26 21 DNA Artificial Sequence Synthetic DNA 26 tctgacaagg cagcaaggtt a 21 27 27 DNA Artificial Sequence Synthetic DNA 27 gatccacttc tggaagcacg tcaaacg 27 28 27 DNA Artificial Sequence Synthetic DNA 28 gggggtaata aaagaatgta tgtcggg 27 29 26 DNA Artificial Sequence Synthetic DNA 29 atgtatgtcg ggcgacataa agctgg 26 30 25 DNA Artificial Sequence Synthetic DNA 30 gatggttggg gggatttcac tgtaa 25 31 27 DNA Artificial Sequence Synthetic DNA 31 ttcactgtaa acgatggggc agtttcg 27 32 21 DNA Artificial Sequence Synthetic DNA 32 ggtttgggtg cagcaataaa t 21 33 33 DNA Artificial Sequence Synthetic DNA 33 tttgaatggc atttgnnnaa tgacgggaac cac 33 34 33 DNA Artificial Sequence Synthetic DNA 34 aaattcaccg gaaagnnntg ggactgggaa gta 33 35 33 DNA Artificial Sequence Synthetic DNA 35 gtgaaacata ttaaannnag ctatacgaga gat 33 36 33 DNA Artificial Sequence Synthetic DNA 36 cttgctgcaa tcgaannnta tttaaataaa aca 33 37 25 DNA Artificial Sequence Synthetic DNA 37 gatggttggg gggaattcac tgtaa 25 38 39 DNA Artificial Sequence Synthetic DNA 38 ccaacgaatc gttggcaggt aatttcaggt gcctacacg 39 39 33 DNA Artificial Sequence Synthetic DNA 39 attgatgcgt ggacgagttt cgacttttca ggg 33 40 21 DNA Artificial Sequence Synthetic DNA 40 tttcgacttt ccagggcgta a 21 41 43 DNA Artificial Sequence Synthetic DNA 41 ggtgttgact gggatcagca atatcaagaa aatcatattt tcc 43 42 42 DNA Artificial Sequence Synthetic DNA 42 catattttcc gctttgcaaa tacggtntgg aacaggcgag tg 42 43 44 DNA Artificial Sequence Synthetic DNA 43 aatatcgact ttagtcatcc agatgtacaa gatgagttga agga 44 44 43 DNA Artificial Sequence Synthetic DNA 44 gacgtaggtg ctctcgaatc ttatttagat gaaatgaatt ggg 43 45 21 DNA Artificial Sequence Synthetic DNA 45 cgataacatt ccagctaaaa a 21 46 38 DNA Artificial Sequence Synthetic DNA 46 gacctggtgg ttccaagaga atgtatgtag gacgtcag 38 47 42 DNA Artificial Sequence Synthetic DNA 47 aatggcgatg gatggggcga tttctttacg aatggaggat ct 42 48 39 DNA Artificial Sequence Synthetic DNA 48 ccaacgaatc gttggcagnn natttcaggt gcctacacg 39 49 33 DNA Artificial Sequence Synthetic DNA 49 gttgactggg atgagcgcaa acaagaaaat cat 33 50 25 DNA Artificial Sequence Synthetic DNA 50 tggatgaaga gttcggtaat tatga 25 51 33 DNA Artificial Sequence Synthetic DNA 51 agtcatccag aggtcgtaga tgagttgaag gat 33 

What is claimed is:
 1. A mutant α-amylase which is derived from an α-amylase consisting of an amino acid sequence represented by SEQ ID No. 2 by substitution or deletion of at least one amino acid residue corresponding to any one of Pro₁₈, Gln₈₆, Glu₁₃₀, Asn₁₅₄, Arg₁₇₁, Ala₁₈₆, Glu₂₁₂, Val₂₂₂, Tyr₂₄₃, Pro₂₆₀, Lys₂₆₉, Glu₂₇₆, Asn₂₇₇, Arg₃₁₀, Glu₃₆₀, Gln₃₉₁, Trp₄₃₉, Lys₄₄₄, Asn₄₇₁, and Gly₄₇₆ of the amino acid sequence.
 2. A mutant α-amylase according to claim 1, wherein the substitution or deletion of at least one amino acid residue is substitution of the amino acid residue corresponding to Pro₁₈ with Ser or Thr, the amino acid residue corresponding to Gln₈₆ with Glu, the amino acid residue corresponding to Glu₁₃₀ with Val or Gln, the amino acid residue corresponding to Asn₁₅₄ with Asp, the amino acid residue corresponding to Arg₁₇₁ with Cys or Gln, the amino acid residue corresponding to Ala₁₈₆ with Val or Asn, the amino acid residue corresponding to Glu₂₁₂ with Asp, the amino acid residue corresponding to Val₂₂₂ with Glu, the amino acid residue corresponding to Tyr₂₄₃ with Cys or Ser, the amino acid residue corresponding to Pro₂₆₀ with Glu, the amino acid residue corresponding to Lys₂₆₉ with Gln, the amino acid residue corresponding to Glu₂₇₆ with His, the amino acid residue corresponding to Asn₂₇₇ with Ser or Phe, the amino acid residue corresponding to Arg₃₁₀ with Ala, the amino acid residue corresponding to Glu₃₆₀ with Gln, the amino acid residue corresponding to Gln₃₉₁ with Glu, the amino acid residue corresponding to TRp4₄₃₉ with Arg, the amino acid residue corresponding to Lys₄₄₄ with Arg, the amino acid residue corresponding to Asn₄₇₁ with Asp or Glu, or the amino acid residue corresponding to Gly₄₇₆ with Asp.
 3. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Pro₁₈ with Ser or Thr.
 4. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Gln₈₆ with Glu.
 5. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Glu₁₃₀ with Val or Gln.
 6. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Asn₁₅₄ with Asp.
 7. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Arg₁₇₁ with Cys or Gln.
 8. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Ala₁₈₆ with Val or Asn.
 9. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Glu₂₁₂ with Asp.
 10. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Val₂₂₂ with Glu.
 11. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Tyr₂₄₃ with Cys or Ser.
 12. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Pro₂₆₀ with Glu.
 13. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Lys₂₆₉ with Gln.
 14. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Glu₂₇₆ with His.
 15. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Asn₂₇₇ with Ser or Phe.
 16. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Arg₃₁₀ with Ala.
 17. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Glu₃₆₀ with Gln.
 18. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Gln₃₉₁ with Glu.
 19. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Trp₄₃₉ with Arg.
 20. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Lys₄₄₄ with Arg.
 21. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Asn₄₇₁ with Asp or Glu.
 22. The mutant α-amylase according to claim 2, wherein the substitution or deletion of at least one amino acid residue is the amino acid residue corresponding to Gly₄₇₆ with Asp.
 23. A composition comprising the mutant α-amylase of claim 1 and a carrier.
 24. A composition comprising the mutant α-amylase of claim 2 and a carrier.
 25. A method of making a detergent, comprising adding the mutant α-amylase of claim 1 to one or more detergent components.
 26. A method of making a detergent, comprising adding the mutant α-amylase of claim 2 to one or more detergent components. 